Isolation, purification and characterization of surface antigens of the bovine filarial parasite Setaria digitata for the immunodiagnosis of bancroftian filariasis.
Identifieur interne : 00DA10 ( Main/Exploration ); précédent : 00DA09; suivant : 00DA11Isolation, purification and characterization of surface antigens of the bovine filarial parasite Setaria digitata for the immunodiagnosis of bancroftian filariasis.
Auteurs : J G Theodore [Inde] ; P. KalirajSource :
- Journal of helminthology [ 0022-149X ] ; 1990.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antihelminthe (analyse), Antigènes d'helminthe (analyse), Antigènes d'helminthe (immunologie), Antigènes d'helminthe (isolement et purification), Antigènes de surface (analyse), Antigènes de surface (immunologie), Antigènes de surface (isolement et purification), Chromatographie d'affinité, Filarioidea (immunologie), Filariose lymphatique (diagnostic), Filarioses (diagnostic), Humains, Immunotransfert, Infection à Setaria (parasitologie), Réactions croisées, Test ELISA, Wuchereria (immunologie), Wuchereria bancrofti (immunologie), Électrophorèse sur gel de polyacrylamide.
- MESH :
- analyse : Anticorps antihelminthe, Antigènes d'helminthe, Antigènes de surface.
- diagnostic : Filariose lymphatique, Filarioses.
- immunologie : Antigènes d'helminthe, Antigènes de surface, Filarioidea, Wuchereria, Wuchereria bancrofti.
- isolement et purification : Antigènes d'helminthe, Antigènes de surface.
- parasitologie : Infection à Setaria.
- Animaux, Chromatographie d'affinité, Humains, Immunotransfert, Réactions croisées, Test ELISA, Électrophorèse sur gel de polyacrylamide.
English descriptors
- KwdEn :
- Animals, Antibodies, Helminth (analysis), Antigens, Helminth (analysis), Antigens, Helminth (immunology), Antigens, Helminth (isolation & purification), Antigens, Surface (analysis), Antigens, Surface (immunology), Antigens, Surface (isolation & purification), Chromatography, Affinity, Cross Reactions, Electrophoresis, Polyacrylamide Gel, Elephantiasis, Filarial (diagnosis), Enzyme-Linked Immunosorbent Assay, Filariasis (diagnosis), Filarioidea (immunology), Humans, Immunoblotting, Setariasis (parasitology), Wuchereria (immunology), Wuchereria bancrofti (immunology).
- MESH :
- chemical , analysis : Antibodies, Helminth, Antigens, Helminth, Antigens, Surface.
- chemical , immunology : Antigens, Helminth, Antigens, Surface.
- chemical , isolation & purification : Antigens, Helminth, Antigens, Surface.
- diagnosis : Elephantiasis, Filarial, Filariasis.
- immunology : Filarioidea, Wuchereria, Wuchereria bancrofti.
- parasitology : Setariasis.
- Animals, Chromatography, Affinity, Cross Reactions, Electrophoresis, Polyacrylamide Gel, Enzyme-Linked Immunosorbent Assay, Humans, Immunoblotting.
Abstract
The surface antigens of the bovine filarial parasite Setaria digitata were isolated by EDTA extraction and purified by affinity chromatography using sepharose bound human filarial (Wuchereria bancrofti) antibodies obtained from chronic human filarial sera. The purified and crude antigens were used in enzyme-linked immunosorbent assay (ELISA) for the detection of serum antibodies in bancroftian filariasis. The purified antigen showed sensitive and specific reactions in ELISA for the detection of antibodies in filarial sera and showed least cross reactivity with other parasitic infections. The crude and purified antigens showed about 18 and 6 peptide bands respectively in SDS-PAGE and about 11 and 6 antigenic bands respectively in enzyme-linked immunoelectrotransfer blot (EITB). The purified antigen was observed to be glycoprotein in nature. It was possible to identify the stage-specific infection in human filariasis by using the crude and purified antigens in EITB.
PubMed: 2201719
Affiliations:
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Kaliraj</name></author></analytic><series><title level="j">Journal of helminthology</title><idno type="ISSN">0022-149X</idno><imprint><date when="1990" type="published">1990</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Helminth (analysis)</term><term>Antigens, Helminth (analysis)</term><term>Antigens, Helminth (immunology)</term><term>Antigens, Helminth (isolation & purification)</term><term>Antigens, Surface (analysis)</term><term>Antigens, Surface (immunology)</term><term>Antigens, Surface (isolation & purification)</term><term>Chromatography, Affinity</term><term>Cross Reactions</term><term>Electrophoresis, Polyacrylamide Gel</term><term>Elephantiasis, Filarial (diagnosis)</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Filariasis (diagnosis)</term><term>Filarioidea (immunology)</term><term>Humans</term><term>Immunoblotting</term><term>Setariasis (parasitology)</term><term>Wuchereria (immunology)</term><term>Wuchereria bancrofti (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps antihelminthe (analyse)</term><term>Antigènes d'helminthe (analyse)</term><term>Antigènes d'helminthe (immunologie)</term><term>Antigènes d'helminthe (isolement et purification)</term><term>Antigènes de surface (analyse)</term><term>Antigènes de surface (immunologie)</term><term>Antigènes de surface (isolement et purification)</term><term>Chromatographie d'affinité</term><term>Filarioidea (immunologie)</term><term>Filariose lymphatique (diagnostic)</term><term>Filarioses (diagnostic)</term><term>Humains</term><term>Immunotransfert</term><term>Infection à Setaria (parasitologie)</term><term>Réactions croisées</term><term>Test ELISA</term><term>Wuchereria (immunologie)</term><term>Wuchereria bancrofti (immunologie)</term><term>Électrophorèse sur gel de polyacrylamide</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Antibodies, Helminth</term><term>Antigens, Helminth</term><term>Antigens, Surface</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antigens, Helminth</term><term>Antigens, Surface</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Antigens, Helminth</term><term>Antigens, Surface</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Anticorps antihelminthe</term><term>Antigènes d'helminthe</term><term>Antigènes de surface</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Elephantiasis, Filarial</term><term>Filariasis</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Filariose lymphatique</term><term>Filarioses</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Antigènes d'helminthe</term><term>Antigènes de surface</term><term>Filarioidea</term><term>Wuchereria</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Filarioidea</term><term>Wuchereria</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Antigènes d'helminthe</term><term>Antigènes de surface</term></keywords><keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Infection à Setaria</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Setariasis</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Chromatography, Affinity</term><term>Cross Reactions</term><term>Electrophoresis, Polyacrylamide Gel</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Humans</term><term>Immunoblotting</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Chromatographie d'affinité</term><term>Humains</term><term>Immunotransfert</term><term>Réactions croisées</term><term>Test ELISA</term><term>Électrophorèse sur gel de polyacrylamide</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The surface antigens of the bovine filarial parasite Setaria digitata were isolated by EDTA extraction and purified by affinity chromatography using sepharose bound human filarial (Wuchereria bancrofti) antibodies obtained from chronic human filarial sera. The purified and crude antigens were used in enzyme-linked immunosorbent assay (ELISA) for the detection of serum antibodies in bancroftian filariasis. The purified antigen showed sensitive and specific reactions in ELISA for the detection of antibodies in filarial sera and showed least cross reactivity with other parasitic infections. The crude and purified antigens showed about 18 and 6 peptide bands respectively in SDS-PAGE and about 11 and 6 antigenic bands respectively in enzyme-linked immunoelectrotransfer blot (EITB). The purified antigen was observed to be glycoprotein in nature. It was possible to identify the stage-specific infection in human filariasis by using the crude and purified antigens in EITB.</div></front></TEI><affiliations><list><country><li>Inde</li></country></list><tree><noCountry><name sortKey="Kaliraj, P" sort="Kaliraj, P" uniqKey="Kaliraj P" first="P" last="Kaliraj">P. Kaliraj</name></noCountry><country name="Inde"><noRegion><name sortKey="Theodore, J G" sort="Theodore, J G" uniqKey="Theodore J" first="J G" last="Theodore">J G Theodore</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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